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| MLO | Mason Lake Ocean | |
| MLO | My Line Overboard | |
| MLO | Medical Laboratory Observer | |
| MLO | Medical Laboratory Observer | |
| MLO | Medical Laboratory Observer | |
| MLO | Major Learning Outcome | |
| MLO | Major Learning Objective | |
| MLO | Mount Laguna Observatory | |
| MLO | Messianic Literature Outreach | |
| MLO | The Mens Liberation Organization | |
| MLO | Malibu Locals Only | |
| MLO | Misce Looney Ous | |
| MLO | Mildew-resistance LOcus gene | |
| MLO | Military Liaison Office | |
| MLO | Mortgage Loan Officer | |
| MLO | Mortgage Loan Originator | |
| MLO | Mauna Loa Observatory | |
| MLO | Mortgage Loan Origination | |
| MLO | Marine Life Observatory | |
| MLO | Model Lighting Ordinance | |
| MLO | Metro Ligero Oeste | |
| MLO | Maria's Lash Out | |
| MLO | Mandriva Linux Online | |
| MLO | My Life Organized | |
| MLO | Materiel Liaison Office |
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