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| O2 | Optimistic Orators Toastmasters Club | |
| O2 SAT | Oxygen SATurated hemoglobin | |
| O20 | Kingdon Airpark, Lodi, California USA | |
| O22 | Columbia Municipal Airport, Columbia, California USA | |
| O26 | Lone Pine Airport, Lone Pine, California USA | |
| O28 | Willits Municipal Airport, Ells Field, Willits, California USA | |
| O2A | Order-to-Activation | |
| O2B | Owner to Business | |
| O2C | Order to Cash | |
| O2CM | Object Class and Contracts Support Model | |
| O2CV | Ostfold 2cv Klubb | |
| O2I | Object-Oriented Interface | |
| O2IC | Officer 2nd In Command | |
| O2K | Office 2000 | |
| O2L | Over Two Liter | |
| O2L | ON2LONDON | |
| O2L | Our Second Life | |
| O2L | Oxygen level in 'spacecraft, spacelab: | |
| O2M | One to Many | |
| O2O | One to One | |
| O2O | Organization to Organization | |
| O2O | One to One | |
| O2O | Online to Offline | |
| O2O | Observe to Obey | |
| O2S | Oxygen Sensor |
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