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| OS | Output Swing | |
| OS | Operational Software | |
| OS | Only So | |
| OS | Opposite Sign | |
| OS | Osteogenic Sarcoma | |
| OS | Odd Squad | |
| OS | Open Software | |
| OS | Oil Spill | |
| OS | Operating System | |
| OS | Origin of Species | |
| OS | Online Seeding | |
| OS | Onside (or Off side) | |
| OS | the Opposite Sex | |
| OS & E | Operating Supplies & Equipment | |
| OS (CDER) | Operations Staff (Center for Drug Evaluation and Research) | |
| OS (CFSAN) | Office of Seafood (CFSAN) | |
| OS (OC) | Operations Staff (Office of the Commissioner) | |
| OS&E | Operating Supplies and Equipment | |
| OS&D | Over, Short, and Damaged | |
| OS-JTF | Open Systems - Joint Task Force | |
| OS/2 | Obsolete Soon, Too | |
| OS/2 | Operating System/2 (IBM-Microsoft) | |
| OS/2 | Operating System/2 | |
| OS/360 | IBM 360 Operating System | |
| OS/360 | IBM 360 operating system (1960s) |
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