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| DFO | Department of Fisheries and Oceans | |
| DFO | Donut Field Officer | |
| DFO | Digital Fuel Optimization | |
| DFO | Dennys Food Organization | |
| DFO | Designer Factory Outlets | |
| DFO | Direct Factory Outlet | |
| DFO | Dark Forcers Organization | |
| DFO | Designated Federal Official | |
| DFO | Disaster Field Office | |
| DFO | Dungeon Fighter Online | |
| DFO | Done Fell Over | |
| DFO | Don't Flip Out | |
| DFO | Department of Fisheries and Oceans (government of Canada) | |
| DFO | Dairy Farmers of Ontario (created August 1, 1995 supplanting the OMMB) | |
| DFO | Dairy Farmers of Ontario | |
| DFO | Distillate Fuel Oil | |
| DFO | Date file opened | |
| DFO | Division of Financial Operations | |
| DFO | Done Fell Out | |
| DFO | Danube Fashion Office | |
| DFO | Derivative Free Optimization | |
| DFO | District Forest Offices | |
| DFO | Desktop Flickr Organizer | |
| DFO | Denver Field Ornithologists | |
| DFO | Dual Filter Oscillator |
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