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| MLRS | Modified Lite Racing Series | |
| MLRS-TGW | Multiple Launch Rocket System Terminally 5 Guided Weapon | |
| MLRT | Macau Light Rapid Transit | |
| MLS | Major League Soccer | |
| MLS | Multi-Level Security | |
| MLS | Miles City, Montana USA | |
| MLS | Mills Corporation | |
| MLS | Multi-Layer Switching | |
| MLS | Microwave Landing System | |
| MLS | Movimiento de Liberación de Septa | |
| MLS | Medical Laboratory Science | |
| MLS | Multiple Listing Service | |
| MLS | Macrolides, Lincosamides, and Streptogra | |
| MLS | Maximum Long String | |
| MLS | Multiple Listing System | |
| MLS | Meyer Listing Systems | |
| MLS | Multiple Licensing Service | |
| MLS | Multiple List Service | |
| MLS | Multi-Level Security | |
| MLS | Modular Lighting System | |
| MLS | Multi Lingual Scholar | |
| MLS | Mac's Last Stand | |
| MLS | Master of Library Science | |
| MLS | Maximum Length Sequence | |
| MLS | Marketing Locating System |
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