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| VLS | Visual Listing Systems | |
| VLS | Villanova Law School | |
| VLS | Volume Level Scaling | |
| VLS | Vascular Leak Syndrome | |
| VLS | Virtual Library System | |
| VLS | Valk Last Slot | |
| VLS | Vapour Liquid Solid | |
| VLS | Velocity Lowest Selectable | |
| VLS | Very Low and Strong | |
| VLS | Variable Launch and Spin | |
| VLS | Visionary Link Service | |
| VLS | Vanguard Logistics Services | |
| VLS | Virtual Learning Solutions | |
| VLS | Virtual Lab School | |
| VLS | Vacuum Lifting Systems | |
| VLS | Ventro-Lateral Septum | |
| VLS | Video Lighting and Sound | |
| VLS | Vehicle Location Service | |
| VLS | Valeo Lighting Systems | |
| VLS | Van with Lift Up and Swing Out Back Door | |
| VLS | Vehicle Level Sensor | |
| VLS | Versata Logic Server | |
| VLS | Variable Length Structure | |
| VLS | Virtual Lan Link State | |
| VLS | Virtual Library Systems |
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