What does FLIM mean in Photography & Imaging?

This page is about the meanings of the acronym/abbreviation/shorthand FLIM in the Miscellaneous field in general and in the Photography & Imaging terminology in particular.

Fluorescence-Lifetime Imaging Microscopy

Miscellaneous » Photography & Imaging

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Submitted by wikidude on July 30, 2019

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Definition

What does FLIM mean?

flim
Fluorescence-lifetime imaging microscopy or FLIM is an imaging technique based on the differences in the exponential decay rate of the photon emission of a fluorophore from a sample. It can be used as an imaging technique in confocal microscopy, two-photon excitation microscopy, and multiphoton tomography. The fluorescence lifetime (FLT) of the fluorophore, rather than its intensity, is used to create the image in FLIM. Fluorescence lifetime depends on the local micro-environment of the fluorophore, thus precluding any erroneous measurements in fluorescence intensity due to change in brightness of the light source, background light intensity or limited photo-bleaching. This technique also has the advantage of minimizing the effect of photon scattering in thick layers of sample. Being dependent on the micro-environment, lifetime measurements have been used as an indicator for pH, viscosity and chemical species concentration.

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