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| DFO | Department of Fisheries and Oceans | |
| DFO | Don't Flip Out | |
| DFO | Don't Freak Out | |
| DFO | Department of Fisheries and Oceans (government of Canada) | |
| DFO | Danube Fashion Office | |
| DFO | Designated Federal Official | |
| DFO | Direct Factory Outlet | |
| DFO | District Forest Offices | |
| DFO | Divisional Forest Officer | |
| DFO | District forest officer | |
| DFO | Dungeon Fighter Online | |
| DFO | Division of Financial Operations | |
| DFO | Department of Fisheries and Oceans | |
| DFO | Data Flow Operator | |
| DFO | Dispatcher Failover | |
| DFO | Derived Format Object | |
| DFO | Data Flow Object | |
| DFO | Dairy Farmers of Ontario (created August 1, 1995 supplanting the OMMB) | |
| DFO | Dennys Food Organization | |
| DFO | Done Fell Over | |
| DFO | Dining Facility Operation | |
| DFO | Disaster Field Office | |
| DFO | Don't Fall Over | |
| DFO | Dartmouth Flood Observatory | |
| DFO | Department of Fisheries and Oceans |
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