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| HLO | Healthcare Loc | |
| HLO | Helicopter Landing Officer | |
| HLO | Hashem Law Office | |
| HLO | Hierarchy of Learner Objectives | |
| HLO | High Low Other | |
| HLO | High Lunar Orbit | |
| HLO | Helicobacter Like Organism | |
| HLO | Hydraulic Lock Out | |
| HLO | Health Licensing Office Health Licensing Office | |
| HLO | HDR Liaison Officer | |
| HLO | High Level Optimization | |
| HLO | Homeless Liaison Officer | |
| HLO | Health Liaison Officer | |
| HLO | Hero Lab Online | |
| HLO | Human Lab Operations | |
| HLO | Hidden Layer Output | |
| HLO | Head of Local Operations | |
| HLO | High Level Orchestrator | |
| HLO | Harmonized Learning Outcomes | |
| HLO | Humber Learning Outcomes | |
| HLO | Holiday Lighting Outlet | |
| HLO | Health Liaison Officers | |
| HLO | High Level Optimizations | |
| HLOC | Higher Level Of Care | |
| HLOF | Homozygous loss of function |
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