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What does FLIM stand for? 

What does FLIM mean? This page is about the various possible meanings of the acronym, abbreviation, shorthand or slang term: FLIM.

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FLIM

Faithful Library About Internet Message

Computing » IT

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FLIM

Fluorescence Lifetime Imaging Microscope

Miscellaneous » Photography & Imaging

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FLIM

Fluorescence Lifetime Imaging Microscopy

Miscellaneous » Photography & Imaging

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FLIM

Fluorescence Live Imaging Microscopy

Miscellaneous » Photography & Imaging

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FLIM

Fluorescence-Lifetime Imaging Microscopy

Miscellaneous » Photography & Imaging

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FLIM

Fluorescent Lifetime Imaging Microscopy

Miscellaneous » Photography & Imaging

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FLIM

Force Level Information Management

Business » Management

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What does FLIM mean?

flim
Fluorescence-lifetime imaging microscopy or FLIM is an imaging technique based on the differences in the exponential decay rate of the photon emission of a fluorophore from a sample. It can be used as an imaging technique in confocal microscopy, two-photon excitation microscopy, and multiphoton tomography. The fluorescence lifetime (FLT) of the fluorophore, rather than its intensity, is used to create the image in FLIM. Fluorescence lifetime depends on the local micro-environment of the fluorophore, thus precluding any erroneous measurements in fluorescence intensity due to change in brightness of the light source, background light intensity or limited photo-bleaching. This technique also has the advantage of minimizing the effect of photon scattering in thick layers of sample. Being dependent on the micro-environment, lifetime measurements have been used as an indicator for pH, viscosity and chemical species concentration.

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